EasySep™ Human IgG+ Memory B Cell Isolation Kit_产品中心

细胞分选产品

EasySep™ Human IgG+ Memory B Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

概要

技术资料

数据及文献

概要

The EasySep™ Human IgG+ Memory B Cell Isolation Kit isolates IgG+CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells, as well as IgM+, IgD+ and IgA+ cells are depleted using EasySep™ Dextran RapidSpheres™. The final isolated fraction contains IgG+CD27+ memory B cells, which readily secrete IgG in culture supernatants in the presence of appropriate stimuli.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Human IgG+ Memory B Cell Isolation Kit	17868	All	English
Safety Data Sheet 1	EasySep™ Human IgG+ Memory B Cell Isolation Kit	17868	All	English
Safety Data Sheet 2	EasySep™ Human IgG+ Memory B Cell Isolation Kit	17868	All	English
Safety Data Sheet 3	EasySep™ Human IgG+ Memory B Cell Isolation Kit	17868	All	English
Safety Data Sheet 4	EasySep™ Human IgG+ Memory B Cell Isolation Kit	17868	All	English
Safety Data Sheet 5	EasySep™ Human IgG+ Memory B Cell Isolation Kit	17868	All	English

数据及文献

Data

Figure 1. EasySep™ Human IgG+ Memory B Cell Isolation Kit

Starting with PBMCs, the IgG+ memory B cell content (CD19+CD27+IgG+) of the isolated fraction is typically 82.6 ± 7.0% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 1.0% and 90.2% respectively.