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EasySep™ Human ILC2 Enrichment Kit

Immunomagnetic negative selection cell enrichment kit

概要
技术资料
数据及文献

概要

The EasySep™ Human ILC2 enrichment kit is designed to enrich Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILC2s and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.

数据及文献

Data


Figure 1. Typical EasySep™ Human ILC2 Enrichment Profile

Starting with washed leukapheresis sample, the ILC2 content (Lin-CD45+CD294+CD127+CD161+) of the enriched fraction typically ranges from 13 - 78%. In the above example, the percentages of ILC2s in the start and final enriched fractions are 0.03% and 35.3%, respectively. NOTE: The ILC2 content of the start fraction typically ranges from 0.001 - 0.16%.