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概要
The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.
This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human EpCAM Positive Selection Kit II | 17846 | All | English |
| Product Information Sheet | RoboSep™ Human EpCAM Positive Selection Kit II | 17846RF | All | English |
| Safety Data Sheet 1 | EasySep™ Human EpCAM Positive Selection Kit II | 17846 | All | English |
| Safety Data Sheet 2 | EasySep™ Human EpCAM Positive Selection Kit II | 17846 | All | English |
| Safety Data Sheet 3 | EasySep™ Human EpCAM Positive Selection Kit II | 17846 | All | English |
| Safety Data Sheet 1 | RoboSep™ Human EpCAM Positive Selection Kit II | 17846RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Human EpCAM Positive Selection Kit II | 17846RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Human EpCAM Positive Selection Kit II | 17846RF | All | English |
数据及文献
Data
Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.
