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概要
The EasySep™ Human Eosinophil Isolation Kit is designed to isolate eosinophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-eosinophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).
This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).
This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Eosinophil Isolation Kit | 17956 | All | English |
| Product Information Sheet | RoboSep™ Human Eosinophil Isolation Kit | 17956RF | All | English |
| Safety Data Sheet 1 | EasySep™ Human Eosinophil Isolation Kit | 17956 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Eosinophil Isolation Kit | 17956 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Eosinophil Isolation Kit | 17956 | All | English |
| Safety Data Sheet 1 | RoboSep™ Human Eosinophil Isolation Kit | 17956RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Human Eosinophil Isolation Kit | 17956RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Human Eosinophil Isolation Kit | 17956RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Eosinophil Isolation Profile
Starting with whole blood prepared using RBC lysis or HetaSep™, the eosinophil content (CD16-CD66b+CD45+) of the isolated fraction is typically 96.5 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above examples, the purities of the start and final isolated fractions prepared using RBC lysis are 1.8% and 97.8% (gated on CD45+), respectively, and when prepared using HetaSep™, the purities are 2.4% and 96.8% (gated on CD45+), respectively.
