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概要
Isolate highly purified CD34+ cells from fresh whole umbilical cord blood (CB) using a simple, two-step procedure. First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (15226C) with antibodies recognizing T cell, B cell and myeloid cell surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C), which contains an antibody recognizing CD34.
This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.
If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.
If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).
This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.
If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.
If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Cord Blood CD34 Positive Selection Kit III | 17897 | All | English |
| Product Information Sheet | RoboSep™ Human Cord Blood CD34 Positive Selection Kit III | 17897RF | All | English |
| Safety Data Sheet 1 | EasySep™ Human Cord Blood CD34 Positive Selection Kit III | 17897 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Cord Blood CD34 Positive Selection Kit III | 17897 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Cord Blood CD34 Positive Selection Kit III | 17897 | All | English |
| Safety Data Sheet 1 | RoboSep™ Human Cord Blood CD34 Positive Selection Kit III | 17897RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Human Cord Blood CD34 Positive Selection Kit III | 17897RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Human Cord Blood CD34 Positive Selection Kit III | 17897RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile
Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 ± 12% (mean ± SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 ± 0.25% (mean ± SD).
