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概要
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD8+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD8+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD8+ T cells that are immediately ready for downstream applications. Effector memory CD8+ T cells can be isolated from the CD62L- fraction.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit | 17869 | All | English |
| Safety Data Sheet 1 | EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit | 17869 | All | English |
| Safety Data Sheet 2 | EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit | 17869 | All | English |
| Safety Data Sheet 3 | EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit | 17869 | All | English |
| Safety Data Sheet 4 | EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit | 17869 | All | English |
| Safety Data Sheet 5 | EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit | 17869 | All | English |
数据及文献
Data
Starting with fresh PBMCs, the central memory CD8+ T cell content (CD3+CD8+CD45RO+CD62L+) of the isolated fraction is typically 86.8 ± 8.4% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD8+CD45RO+CD62L-) of the isolated fraction is typically 88.7 ± 6.5% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.
