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EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

概要

技术资料

数据及文献

概要

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English
Safety Data Sheet 1	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English
Safety Data Sheet 2	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English
Safety Data Sheet 3	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English
Safety Data Sheet 4	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English
Safety Data Sheet 5	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English
Safety Data Sheet 6	EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit	17865	All	English

数据及文献

Data

Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.