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EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit

25-Minute cell isolation kit using immunomagnetic negative selection

概要
技术资料
数据及文献

概要

The EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This kit has been verified for use with rhesus and cynomolgus macaques.

数据及文献

Data

The content of rhesus macaque CD8+ T cells before and after isolation correspond to 55.7% and 93.9%, respectively.

Figure 1. EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit

In this example, starting with rhesus macaque PBMCs, the CD8+ T cell content (within the CD45+CD3+ gate) of start and final isolated fractions are 50.7% and 94.1%, respectively.