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概要
The EasySep™ Total Lymphocyte Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human lymphocytes from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD14, CD15, CD16, CD33, CD36, CD41, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
技术资料
数据及文献
Data
Figure 1. Typical EasySep™ Total Lymphocyte Enrichment Profile
Starting with HetaSep™-treated whole blood, the CD3+ / CD19+ cell content of the enriched fraction typically ranges from 90.2 - 96.9% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.3 - 3.6 x 10e6 CD3+ or CD19+ cells are typically recovered.
