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概要
The EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit is designed to isolate highly purified CD33+ and CD66b+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens is expressed on myeloid progenitor cells, granulocytes and monocytes.
This product is an improved version of the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) that provides highly purified cells using a shorter protocol.
This product replaces the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA), for even faster cell isolations.
This product is an improved version of the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) that provides highly purified cells using a shorter protocol.
This product replaces the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA), for even faster cell isolations.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884 | All | English |
| Product Information Sheet | RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884RF | All | English |
| Safety Data Sheet 1 | EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884 | All | English |
| Safety Data Sheet 2 | EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884 | All | English |
| Safety Data Sheet 3 | EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884 | All | English |
| Safety Data Sheet 1 | RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884RF | All | English |
| Safety Data Sheet 2 | RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884RF | All | English |
| Safety Data Sheet 3 | RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit | 17884RF | All | English |
数据及文献
Data
Starting with human whole blood, the myeloid cell content of the isolated fraction is typically 94.5 ± 4.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 66.6% and 98.5%, respectively.
