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概要
The EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit is designed to isolate highly purified myeloid (including CD14+ and CD66b+) cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on myeloid progenitor cells, granulocytes and monocytes.
This product replaces the EasySep™ Human Whole Blood CD33 Positive Selection Kit (Catalog #18287) and EasySep™ HLA Whole Blood CD33 Positive Selection Kit (Catalog #18287HLA) for even faster cell isolations.
This product replaces the EasySep™ Human Whole Blood CD33 Positive Selection Kit (Catalog #18287) and EasySep™ HLA Whole Blood CD33 Positive Selection Kit (Catalog #18287HLA) for even faster cell isolations.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885 | All | English |
| Product Information Sheet | RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885RF | All | English |
| Safety Data Sheet 1 | EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885 | All | English |
| Safety Data Sheet 2 | EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885 | All | English |
| Safety Data Sheet 3 | EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885 | All | English |
| Safety Data Sheet 1 | RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885RF | All | English |
| Safety Data Sheet 2 | RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885RF | All | English |
| Safety Data Sheet 3 | RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit | 17885RF | All | English |
数据及文献
Data
Starting with fresh whole blood, the CD33+ cell content of the isolated fraction is typically 96 ± 2% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start (as assessed by labeling with anti-CD33) and the final isolated fractions are 68.0% and 96.2% (as assessed by labeling with anti-CD14 and anti-CD66b), respectively.
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.
