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概要
The EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells.
This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.
This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.
技术资料
数据及文献
Data
Starting with human whole blood, the B cell content (CD19+CD20+) of the isolated fraction is typically 92.5 ± 5.6% (gated on CD45+ cells; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 92.2%, respectively.
