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EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

概要

技术资料

数据及文献

概要

The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.

This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet 1	EasySep™ HLA Chimerism BC CD56 Positive Selection Kit	17875	All	English
Product Information Sheet 2	EasySep™ HLA Chimerism BC CD56 Positive Selection Kit	17875	All	English
Product Information Sheet 1	RoboSep™ HLA Chimerism BC CD56 Positive Selection Kit	17875RF	All	English
Product Information Sheet 2	RoboSep™ HLA Chimerism BC CD56 Positive Selection Kit	17875RF	All	English
Safety Data Sheet 1	EasySep™ HLA Chimerism BC CD56 Positive Selection Kit	17875	All	English
Safety Data Sheet 2	EasySep™ HLA Chimerism BC CD56 Positive Selection Kit	17875	All	English
Safety Data Sheet 1	RoboSep™ HLA Chimerism BC CD56 Positive Selection Kit	17875RF	All	English
Safety Data Sheet 2	RoboSep™ HLA Chimerism BC CD56 Positive Selection Kit	17875RF	All	English

数据及文献

Data

Starting with freshly prepared buffy coat, the CD56+ cell content of the isolated fraction typically ranges from 95.8 - 99.5% (gated on CD45+ using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.5% and 98.4%, respectively. NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.