EasySep™ Direct Human T Cell Isolation Kit_产品中心_北京砺达辰生物科技有限公司

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EasySep™ Direct Human T Cell Isolation Kit

Immunomagnetic negative selection from whole blood kit

概要

技术资料

数据及文献

概要

This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.

This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Direct Human T Cell Isolation Kit	19661	18F92420 or higher	English
Product Information Sheet	EasySep™ Direct Human T Cell Isolation Kit for RoboSep™	19661RF	18F92420 or higher	English
Safety Data Sheet 1	EasySep™ Direct Human T Cell Isolation Kit	19661	All	English
Safety Data Sheet 2	EasySep™ Direct Human T Cell Isolation Kit	19661	All	English

数据及文献

Data

Isolated T cells from whole blood obtained using EasySep™ Direct human T cell isolation kit

Figure 1. Typical EasySep™ Direct Human T Cell Isolation Profile

Starting with human whole blood from normal healthy donors, the typical T cell (CD3+) content of the non-lysed final isolated fraction is 95.3 ± 1.4% (gated on CD45) or 94.9 ± 1.5% (not gated on CD45). In the example above, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 33.0% and 95.0% (gated on CD45), respectively, or 33.0% and 94.9% (not gated on CD45), respectively. The starting frequency of T cells in the non-lysed whole blood start sample above is 0.059% (data not shown).