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概要
The EasySep™ Direct Human NK Cell Isolation Kit is designed to isolate NK cells from human whole blood by immunomagnetic negative selection. The EasySep™ Direct procedure involves labeling RBCs, platelets and unwanted cells present in human whole blood with antibody complexes and magnetic particles. The magnetically labeled RBCs, platelets and cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Direct Human NK Cell Isolation Kit | 19665 | All | English |
| Safety Data Sheet 1 | EasySep™ Direct Human NK Cell Isolation Kit | 19665 | All | English |
| Safety Data Sheet 2 | EasySep™ Direct Human NK Cell Isolation Kit | 19665 | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Direct Human NK Cell Isolation Profile
In the above example, the NK cell (CD3-CD56+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 10.6% and 80.0% (gated on CD45), respectively, or 10.5% and 79.7% (not gated on CD45), respectively. The starting frequency of NK cells in the non-lysed whole blood start sample above is 0.011% (data not shown).
