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EasySep™ Direct HLA Crossmatch T Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen or lymph node kit

概要

技术资料

数据及文献

概要

The EasySep™ Direct HLA Crossmatch T Cell Isolation Kit can be used to isolate highly purified T cells directly from human whole blood, spleen or lymph nodes by immunomagnetic negative selection. This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry crossmatch.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Direct HLA Crossmatch T Cell Isolation Kit	19671	18F92320 or higher	English
Product Information Sheet	EasySep™ Direct HLA Crossmatch T Cell Isolation Kit for RoboSep™	19671RF	18F92320 or higher	English
Safety Data Sheet 1	EasySep™ Direct HLA Crossmatch T Cell Isolation Kit	19671	All	English
Safety Data Sheet 2	EasySep™ Direct HLA Crossmatch T Cell Isolation Kit	19671	All	English
Safety Data Sheet 1	EasySep™ Direct HLA Crossmatch T Cell Isolation Kit for RoboSep™	19671RF	All	English
Safety Data Sheet 2	EasySep™ Direct HLA Crossmatch T Cell Isolation Kit for RoboSep™	19671RF	All	English

数据及文献

Data

Starting with human whole blood from normal healthy donors, the T cell (CD3+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.9% (gated on CD45). In the above example, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 38.0% and 93.2% (gated on CD45), respectively.