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概要
The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Direct HLA Crossmatch B Cell Isolation Kit | 19684 | 18F92330 or higher | English |
| Product Information Sheet | EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ | 19684RF | 18F92330 or higher | English |
| Safety Data Sheet 1 | EasySep™ Direct HLA Crossmatch B Cell Isolation Kit | 19684 | All | English |
| Safety Data Sheet 2 | EasySep™ Direct HLA Crossmatch B Cell Isolation Kit | 19684 | All | English |
| Safety Data Sheet 1 | EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ | 19684RF | All | English |
| Safety Data Sheet 2 | EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ | 19684RF | All | English |
数据及文献
Data
Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8% (gated on CD45). In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.
