网站首页
概要
EasySep™ Serology Whole Blood CD19 Positive Selection Kit is designed to isolate highly purified CD19+ cells from fresh human whole blood by immunomagnetic positive selection. This kit targets human CD19+ cells for positive selection with an antibody recognizing the CD19 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as HLA serology, flow cytometry, or DNA/RNA extraction.
技术资料
Scientific Resources
Product Documentation
数据及文献
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
数据
Figure 1. EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Starting with human whole blood, the CD19+ cell content of the isolated fraction typically ranges from 89.4 - 97.8% (note: as the CD19 antibody used for flow cytometry staining was fully blocked by the CD19 antibody used to isolate the cells, staining of B cells was assessed by labeling with anti-CD20 antibody; gated on CD45+). In the above example, the purities of the start and final isolated fractions are 7.3% and 96.2%, respectively (gated on CD45+).
NOTE: Red blood cells were removed from start sample by lysis prior to flow cytometry.
NOTE: The magnetic particles present on the surface of the isolated CD19+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer. This will make it difficult to gate on a specific lymphocyte population based on FSC/SSC. Instead, we recommend first gating the cells on CD45 and examining the lymphocyte populations based on the CD45+ gate.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
