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概要
技术资料
Scientific Resources
Product Documentation
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet 1 | EasySep™ Serology Whole Blood CD3 Positive Selection Kit | 18981 | 1000064545 and lower | English |
| Product Information Sheet 2 | EasySep™ Serology Whole Blood CD3 Positive Selection Kit | 18981 | 1000074633 and higher | English |
| Product Information Sheet 1 | RoboSep™ Serology Whole Blood CD3 Positive Selection Kit | 18981RF | 1000064545 and lower | English |
| Product Information Sheet 2 | RoboSep™ Serology Whole Blood CD3 Positive Selection Kit | 18981RF | 1000074633 and higher | English |
| Safety Data Sheet 1 | EasySep™ Serology Whole Blood CD3 Positive Selection Kit | 18981 | 全部 | English |
| Safety Data Sheet 2 | EasySep™ Serology Whole Blood CD3 Positive Selection Kit | 18981 | 全部 | English |
| Safety Data Sheet 1 | RoboSep™ Serology Whole Blood CD3 Positive Selection Kit | 18981RF | 全部 | English |
| Safety Data Sheet 2 | RoboSep™ Serology Whole Blood CD3 Positive Selection Kit | 18981RF | 全部 | English |
数据及文献
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
数据
Figure 1. EasySep™ Serology Whole Blood CD3 Positive Selection Kit
Starting with human whole blood, the CD3+ cell content of the isolated fraction typically ranges from 96.7 - 99.6% (note: as the CD3 antibody used for flow cytometry staining was fully blocked by the CD3 antibody used to isolate the cells, staining of T cells was assessed by labeling with anti-CD2; gated on CD45+). In the above example, the purities of the start and final isolated fractions are 25.4% and 98.7% (gated on CD45+), respectively.
NOTE: Red blood cells were removed by lysis prior to flow cytometry.
NOTE: The magnetic particles present on the surface of the isolated CD3+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer. This will make it difficult to gate on a specific lymphocyte population based on FSC/SSC. Instead, we recommend first gating the cells on CD45 and examining the lymphocyte populations based on the CD45+ gate.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
