概要
The StemSep™ Human CD14 Positive Selection Kit is designed to isolate CD14+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using commercially available columns and StemSep™ magnets. The CD14 antigen is strongly expressed on monocytes and weakly expressed on granulocytes. It is also expresed on most tissue macrophages.
技术资料
Document Type | 产品名称 | Catalog # | Lot # | 语言 |
---|---|---|---|---|
Product Information Sheet | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
Manual | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
Safety Data Sheet 1 | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
Safety Data Sheet 2 | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
数据及文献
Data
Figure 1. Typical StemSep™ CD14 Selection Profile
The expected CD14+ content of the enriched fraction is 97.5 ± 0.5% with expected recoveries of 58 ± 9%.