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概要
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.
Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail | 15226, 15266 | All | English |
| Safety Data Sheet | RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail | 15226, 15266 | All | English |
数据及文献
Data
Figure 1.Typical RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Profile
Starting with fresh human cord blood, the CD34+CD45+ viable cell content of the pre-enriched fraction typically ranges from 2 - 16%
