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RosetteSep™ Human Cord Blood Debulking Cocktail

Immunodensity depletion cocktail

概要
技术资料
数据及文献

概要

The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.

技术资料

数据及文献

Data

FACS Profile Results with RosetteSep™ De-Bulking Cocktail

Figure 1. FACS Profile Results with RosetteSep™ De-Bulking Cocktail

Starting with fresh cord blood, the enrichment of CD34+ cells is typically 5 ± 1%.