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概要
The RosetteSep™ HLA T Cell Enrichment Cocktail is designed to isolate T cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes (TAC) recognizing non-T cells and red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified T cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The desired cells are immediately ready for serology or flow cytometry crossmatch assays. This kit carries the CE mark and is available as a Class I in vitro diagnostic device in the European Union and Australia. To learn more about the regulatory status of this product in your specific region, contact info@stemcell.com.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet 1 | RosetteSep™ HLA T Cell Enrichment Cocktail | 15061HLA, 15081HLA | All | MULTI |
| Product Information Sheet 2 | RosetteSep™ HLA T Cell Enrichment Cocktail | 15061HLA, 15081HLA | All | English |
| Safety Data Sheet | RosetteSep™ HLA T Cell Enrichment Cocktail | 15061HLA, 15081HLA | All | English |
数据及文献
Data
Figure 1. Typical RosetteSep™ HLA T Cell Enrichment Profile
Starting with fresh whole blood the CD3+ cell content of the enriched fraction typically ranges from 90% - 97%. Red blood cells were removed by lysis prior to flow cytometry.
