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概要
The EasySep™ Release Mouse Biotin Positive Selection Kit is designed to isolate cells that are labeled with biotin-conjugated antibodies from mouse tissue by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556), providing highly purified particle-free cells.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556), providing highly purified particle-free cells.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet 1 | EasySep™ Release Mouse Biotin Positive Selection Kit | 17655 | 19D102085 or lower | English |
| Product Information Sheet 2 | EasySep™ Release Mouse Biotin Positive Selection Kit | 17655 | 1000006156 and higher | English |
| Safety Data Sheet 1 | EasySep™ Release Mouse Biotin Positive Selection Kit | 17655 | All | English |
| Safety Data Sheet 2 | EasySep™ Release Mouse Biotin Positive Selection Kit | 17655 | All | English |
| Safety Data Sheet 3 | EasySep™ Release Mouse Biotin Positive Selection Kit | 17655 | All | English |
| Safety Data Sheet 4 | EasySep™ Release Mouse Biotin Positive Selection Kit | 17655 | All | English |
数据及文献
Data
Starting with fresh PBMCs, the purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody and EasySep™ Release Human Biotin Positive Selection Kit (as assessed by labeling with CD45RO and CD45RA).
Starting with mouse splenocytes, the purities of the start and final isolated fractions are 58.4% and 95.1%, respectively, using a biotinylated anti-mouse CD19 antibody and EasySep™ Release Mouse Biotin Positive Selection Kit (as assessed by labeling with CD19 and CD45R/B220).
