概要
The EasySep™ Release Mouse APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from mouse tissue by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
技术资料
Document Type | 产品名称 | Catalog # | Lot # | 语言 |
---|---|---|---|---|
Product Information Sheet | EasySep™ Release Mouse APC Positive Selection Kit | 100-0033 | All | English |
Safety Data Sheet 1 | EasySep™ Release Mouse APC Positive Selection Kit | 100-0033 | All | English |
Safety Data Sheet 2 | EasySep™ Release Mouse APC Positive Selection Kit | 100-0033 | All | English |
Safety Data Sheet 3 | EasySep™ Release Mouse APC Positive Selection Kit | 100-0033 | All | English |
Safety Data Sheet 4 | EasySep™ Release Mouse APC Positive Selection Kit | 100-0033 | All | English |
数据及文献
Data
Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit
In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.