EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit_产品中心

细胞分选产品

EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

概要

技术资料

数据及文献

概要

The EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit is designed to isolate CD271⁺ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.

Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit	100-0047	All	English
Safety Data Sheet 1	EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit	100-0047	All	English
Safety Data Sheet 2	EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit	100-0047	All	English
Safety Data Sheet 3	EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit	100-0047	All	English
Safety Data Sheet 4	EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit	100-0047	All	English

数据及文献

Data

$A H9 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 74.9% and 93.8% pure.$

Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

$A H7 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 47.3% and 90.8% pure.$

Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

$A 1C cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 18.9% and 86.7% pure.$

Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).