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概要
The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | RoboSep™ Release Human CD4 Positive Selection Kit | 17752RF | All | English |
| Product Information Sheet | EasySep™ Release Human CD4 Positive Selection Kit | 17752 | All | English |
| Safety Data Sheet 1 | RoboSep™ Release Human CD4 Positive Selection Kit | 17752RF | All | English |
| Safety Data Sheet 2 | RoboSep™ Release Human CD4 Positive Selection Kit | 17752RF | All | English |
| Safety Data Sheet 3 | RoboSep™ Release Human CD4 Positive Selection Kit | 17752RF | All | English |
| Safety Data Sheet 1 | EasySep™ Release Human CD4 Positive Selection Kit | 17752 | All | English |
| Safety Data Sheet 2 | EasySep™ Release Human CD4 Positive Selection Kit | 17752 | All | English |
| Safety Data Sheet 3 | EasySep™ Release Human CD4 Positive Selection Kit | 17752 | All | English |
数据及文献
Data
Starting with a single-cell suspension of human PBMCs, the CD4+ T cell content of the isolated fraction is typically 96.1 ± 4.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 24.0% and 98.1%, respectively.
