概要
The EasySep™ Rat B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
技术资料
Document Type | 产品名称 | Catalog # | Lot # | 语言 |
---|---|---|---|---|
Product Information Sheet | EasySep™ Rat B Cell Isolation Kit | 19644 | All | English |
Product Information Sheet | RoboSep™ Rat B Cell Isolation Kit | 19644RF | All | English |
Safety Data Sheet 1 | EasySep™ Rat B Cell Isolation Kit | 19644 | All | English |
Safety Data Sheet 2 | EasySep™ Rat B Cell Isolation Kit | 19644 | All | English |
Safety Data Sheet 1 | RoboSep™ Rat B Cell Isolation Kit | 19644RF | All | English |
Safety Data Sheet 2 | RoboSep™ Rat B Cell Isolation Kit | 19644RF | All | English |
数据及文献
Data
Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.