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EasySep™ Non-Human Primate B Cell Isolation Kit

Immunomagnetic negative selection kit

概要

技术资料

数据及文献

概要

The EasySep™ Non-Human Primate B Cell Isolation Kit is designed to isolate untouched and highly purified B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) in as little as 20 minutes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This kit has been verified for use with rhesus and cynomolgus macaques.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Non-Human Primate B Cell Isolation Kit	100-0345	All	English
Product Information Sheet	RoboSep™ Non-Human Primate B Cell Isolation Kit	100-0347	All	English
Safety Data Sheet 1	EasySep™ Non-Human Primate B Cell Isolation Kit	100-0345	All	English
Safety Data Sheet 2	EasySep™ Non-Human Primate B Cell Isolation Kit	100-0345	All	English
Safety Data Sheet 3	EasySep™ Non-Human Primate B Cell Isolation Kit	100-0345	All	English
Safety Data Sheet 1	RoboSep™ Non-Human Primate B Cell Isolation Kit	100-0347	All	English
Safety Data Sheet 2	RoboSep™ Non-Human Primate B Cell Isolation Kit	100-0347	All	English
Safety Data Sheet 3	RoboSep™ Non-Human Primate B Cell Isolation Kit	100-0347	All	English
Safety Data Sheet 4	RoboSep™ Non-Human Primate B Cell Isolation Kit	100-0347	All	English

数据及文献

Data

The content of rhesus macaque CD20+ B cells before and after isolation correspond to 10.4% and 94.8%, respectively.

Figure 1. EasySep™ Non-Human Primate B Cell Isolation Kit

Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.