EasySep™ Mouse TIL (CD45) Positive Selection Kit_产品中心

概要

The EasySep™ Mouse TIL (CD45) Positive Selection Kit is designed to isolate highly purified CD45+ tumor infiltrating leukocytes (TILs) by immunomagnetic positive selection. This kit has been optimized for use on single-cell suspensions of solid mouse tumors with a low CD45+ start cell frequency, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization.

This kit targets CD45+ cells for positive selection with antibodies recognizing the CD45 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Mouse TIL (CD45) Positive Selection Kit	100-0350	All	English
Safety Data Sheet 1	EasySep™ Mouse TIL (CD45) Positive Selection Kit	100-0350	All	English
Safety Data Sheet 2	EasySep™ Mouse TIL (CD45) Positive Selection Kit	100-0350	All	English
Safety Data Sheet 3	EasySep™ Mouse TIL (CD45) Positive Selection Kit	100-0350	All	English
Safety Data Sheet 4	EasySep™ Mouse TIL (CD45) Positive Selection Kit	100-0350	All	English

数据及文献

Data

$Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.$ $Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.$ $Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.$

Figure 1. EasySep™ Mouse TIL (CD45) Positive Selection Kit

Tumors were induced by B16-F10, 4T1, or CT26.WT cell lines and dissociated into single-cell suspensions. CD45+ TILs were isolated from single-cell suspensions at various start concentrations using the purple EasySep™ Magnet. (A) Starting with a B16-F10 tumor single-cell suspension at 1 x 10^8 cells/mL, the purities of the start and final isolated fractions are 14.8% and 95.2%, respectively. (B) Starting with a 4T1 tumor single-cell suspension at 4 x 10^7 cells/mL, the purities of the start and final isolated fractions are 37.6% and 93.0%, respectively. (C) Starting with a CT26.WT tumor single-cell suspension at 2.5 x 10^7 cells/mL, the purities of the start and final isolated fractions are 32.3% and 84.6%, respectively.