网站首页
概要
This product replaces the EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19763), for improved yield and even faster cell isolations.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Mouse Pan-DC Enrichment Cocktail II | 19863 | All | English |
| Product Information Sheet | RoboSep™ Mouse Pan-DC Enrichment Kit II | 19863RF | All | English |
| Safety Data Sheet | EasySep™ Mouse Pan-DC Enrichment Cocktail II | 19863 | All | English |
| Safety Data Sheet | RoboSep™ Mouse Pan-DC Enrichment Kit II | 19863RF | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile
Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.
Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time
Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).
