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EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit

18-Minute cell isolation kit using immunomagnetic negative selection

概要

技术资料

数据及文献

概要

The EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit is designed to isolate CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-MDSCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell sorting, and cell culture.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit	19867	All	English
Safety Data Sheet 1	EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit	19867	All	English
Safety Data Sheet 2	EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit	19867	All	English

数据及文献

Data

Figure 1. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from 4T1 Tumor-Bearing BALB/c Mouse Splenocytes

Starting with 4T1 tumor-bearing BALB/c mouse splenocytes, the MDSC content (CD11b+Gr1+) of the isolated fraction is typically 94.3 ± 2.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 20.7% and 94.7%, respectively.

Figure 2. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from Naïve C57BL/6 Mouse Splenocytes

Starting with naïve C57BL/6 mouse splenocytes, the CD11b+Gr1+ cell content of the isolated fraction is typically 86 ± 4.6% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 3.0% and 86.8%, respectively.