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EasySep™ Mouse CD90.1 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

概要
技术资料
数据及文献

概要

The EasySep™ Mouse CD90.1 Positive Selection Kit is designed to isolate CD90.1+ (Thy1.1+) cells from single suspensions of splenocytes, lymph nodes and peripheral blood by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.1 and dextran-coated magnetic particles. Labelled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

数据及文献

Data

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Figure 1. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Starting with mouse splenocytes, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 93.5 ± 3.9% (mean ± SD; using the purple EasySep™ Magnet).

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Figure 2. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Starting with mouse splenocytes from adoptive lymphocyte transfer samples, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 90.8 ± 1.5% (mean ± SD; using the purple EasySep™ Magnet).