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概要
The EasySep™ Mouse CD45 Positive Selection Kit is designed to isolate CD45 cells from single-cell suspensions of splenocytes, lung, or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | EasySep™ Mouse CD45 Positive Selection Kit | 18945 | All | English |
| Safety Data Sheet 1 | EasySep™ Mouse CD45 Positive Selection Kit | 18945 | All | English |
| Safety Data Sheet 2 | EasySep™ Mouse CD45 Positive Selection Kit | 18945 | All | English |
| Safety Data Sheet 3 | EasySep™ Mouse CD45 Positive Selection Kit | 18945 | All | English |
数据及文献
Data
Figure 1. Typical EasySep™ Mouse CD45 Positive Selection Profile
(A) Starting with a naïve mouse lung single-cell suspension, the leukocyte content (CD45+) of the isolated fraction is typically 97.0% ± 1.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions in lung are 53.1% and 94.1%, respectively. (B) Starting with unlysed naïve mouse splenocytes, the leukocyte content (CD45+) of the isolated fraction is typically 97.6 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example using spleen, the purities of the start and final isolated fractions are 31.2% and 96.6%, respectively.
