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概要
GloCell™ Fixable Viability Dye Red 780 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | GloCell™ Fixable Viability Dye Red 780 | 75007, 75007.1 | All | English |
| Safety Data Sheet | GloCell™ Fixable Viability Dye Red 780 | 75007, 75007.1 | All | English |
数据及文献
Data
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Red 780. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Red 780.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Red 780. Excitation max: 757 nm, Emission Max: 779 nm
