网站首页
概要
GloCell™ Fixable Viability Dye Red 710 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be used prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Red 710 is excited by the red laser (633 nm) with an excitation maximum of 647 nm, and it has a fluorescence emission maximum of 710 nm that can be detected using the 710/50 band pass filter used to detect Alexa Fluor® 700.
GloCell™ Fixable Viability Dye Red 710 is excited by the red laser (633 nm) with an excitation maximum of 647 nm, and it has a fluorescence emission maximum of 710 nm that can be detected using the 710/50 band pass filter used to detect Alexa Fluor® 700.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | GloCell™ Fixable Viability Dye Red 710 | 75006, 75006.1 | All | English |
| Safety Data Sheet | GloCell™ Fixable Viability Dye Red 710 | 75006, 75006.1 | All | English |
数据及文献
Data
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Red 710. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Red 710.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Red 710. Excitation max: 693 nm, Emission Max: 713 nm
