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概要
Phalloidin is a heptapeptide derived from the poisonous mushroom Amanita phalloides. Phalloidin binds with a high specificity to F-actin filaments in cells, preventing filament depolymerization. Fluorescently labeled phalloidin can be used in labeling, identification, and quantitation of F-actin in formaldehyde-fixed and permeabilized cells. Fluorescently labeled phalloidin has lower non-specific binding compared to actin antibodies across most species of plants and animals, which provides low background and high contrast during imaging.
技术资料
Scientific Resources
Product Documentation
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Phalloidin, iFluor™ 488 | 100-0852 | All | English |
| Product Information Sheet | Phalloidin, iFluor™ 555 | 100-0853 | All | English |
| Product Information Sheet | Phalloidin, iFluor™ 647 | 100-0854 | All | English |
| Safety Data Sheet | Phalloidin, iFluor™ 488 | 100-0852 | 全部 | English |
| Safety Data Sheet | Phalloidin, iFluor™ 555 | 100-0853 | 全部 | English |
| Safety Data Sheet | Phalloidin, iFluor™ 647 | 100-0854 | 全部 | English |
数据及文献
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
数据
Figure 1. Phalloidin-iFluor™ Can Be Used to Stain Embryonic Stem Cells
H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).
Figure 2. Phalloidin-iFluor™ Can Be Used to Stain Forebrain-Type Neurons
WLS-1C cell-derived neural progenitor cells were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation (Catalog #08600) and STEMdiff™ Forebrain Neuron Maturation Kits (Catalog #08605). Neurons were cultured on poly-L-ornithine/laminin-coated 35 mm glass-bottom dishes for 3 weeks, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL
