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细胞染料和检测试剂盒

LysoBrite™ Lysosome Dyes

Fluorescent dyes used to label and track lysosomes in live cells

概要
技术资料
数据及文献

概要

LysoBrite™ dyes are fluorescent dyes that selectively stain lysosomes and other acidic organelles. Due to their hydrophobic nature, LysoBrite™ dyes permeate live cells and selectively accumulate in the lysosomes via the lysosome’s pH gradient. The fluorescence property of these dyes significantly increases within the lysosome’s acidic environment; this fluorescence can be measured using flow cytometry and fluorescence imaging. Due to their selective fluorescence inside lysosomes, LysoBrite™ dyes provide low background and high contrast during imaging. They can be used with proliferating and non-proliferating cells, and are suitable for suspension and adherent cells.

数据及文献

Data and Publications

数据

Image of H9 embryonic stem cells stained with LysoBrite™ Lysosome Dye.

Figure 1. LysoBrite™ Lysosome Dyes Can Be Used to Stain Embryonic Stem Cells

H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then stained with LysoBrite™ Lysosome Dye, Green (green), and counterstained with Hoechst 33342 (blue).