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概要
LysoBrite™ dyes are fluorescent dyes that selectively stain lysosomes and other acidic organelles. Due to their hydrophobic nature, LysoBrite™ dyes permeate live cells and selectively accumulate in the lysosomes via the lysosome’s pH gradient. The fluorescence property of these dyes significantly increases within the lysosome’s acidic environment; this fluorescence can be measured using flow cytometry and fluorescence imaging. Due to their selective fluorescence inside lysosomes, LysoBrite™ dyes provide low background and high contrast during imaging. They can be used with proliferating and non-proliferating cells, and are suitable for suspension and adherent cells.
技术资料
Scientific Resources
Product Documentation
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | LysoBrite™ Lysosome Dye, Deep Red | 100-0819 | All | English |
| Product Information Sheet | LysoBrite™ Lysosome Dye, Red DND-99 | 100-0850 | All | English |
| Product Information Sheet | LysoBrite™ Lysosome Dye, Green | 100-0851 | All | English |
| Safety Data Sheet | LysoBrite™ Lysosome Dye, Deep Red | 100-0819 | 全部 | English |
| Safety Data Sheet | LysoBrite™ Lysosome Dye, Red DND-99 | 100-0850 | 全部 | English |
| Safety Data Sheet | LysoBrite™ Lysosome Dye, Green | 100-0851 | 全部 | English |
数据及文献
Data and Publications
数据
Figure 1. LysoBrite™ Lysosome Dyes Can Be Used to Stain Embryonic Stem Cells
H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then stained with LysoBrite™ Lysosome Dye, Green (green), and counterstained with Hoechst 33342 (blue).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL
