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概要
ES-Cult™ M3120 is recommended as a base for the preparation of methylcellulose-based medium for the in vitro differentiation of mouse embryonic stem (ES) or induced pluripotent stem (iPS) cells into hematopoietic or endothelial progenitor cells. When supplemented with the appropriate additional components, ES-Cult™ M3120 can be used for the generation of embryoid bodies (EBs) from undifferentiated mouse ES or iPS cells.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | ES-Cult™ M3120 | 03120 | All | English |
| Manual | ES-Cult™ M3120 | 03120 | All | English |
| Safety Data Sheet | ES-Cult™ M3120 | 03120 | All | English |
数据及文献
Publications (1)
Yearbook of Dermatology and Dermatologic Surgery 2008 DEC
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Abstract
Abstract
Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.
