ClonaCell™-CHO ACF Supplement_产品中心_北京砺达辰生物科技有限公司

细胞培养基及添加物

ClonaCell™-CHO ACF Supplement

Animal component-free, serum-free, glutamine-free 40X supplement for cloning during CHO cell line development

概要

技术资料

数据及文献

概要

ClonaCell™-CHO ACF Supplement is a serum-free and animal component-free supplement that is added to protein-free media in order to increase cloning efficiency and promote robust growth of CHO cells. The 40X supplement can be added to a variety of protein-free liquid or semi-solid cell culture media to increase the growth and single-cell cloning efficiency of suspension-adapted CHO cells. The supplement contains recombinant proteins and phenol red, and does not contain animal components, serum, hydrolysates, L-glutamine, or selection agents.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	ClonaCell™-CHO ACF Supplement	03820	All	English
Safety Data Sheet	ClonaCell™-CHO ACF Supplement	03820	All	English

数据及文献

Data

Cloning efficiencies of CHO-S and CHO-K1 cells in protein-free media from different commercial suppliers with and without addition of ClonaCell™-CHO ACF Supplement

Figure 1. Cloning efficiencies of CHO-S and CHO-K1 cells in protein-free media from different commercial suppliers with and without addition of ClonaCell™-CHO ACF Supplement

The bar graphs illustrate the cloning efï¬ciency of untransfected CHO-S (A) and CHO-K1 (B) cells, deï¬ned as the percentage of wells in 96-well plates that contain greater than 100 cells after 14 days of incubation. Individual wells of a 96-well plate were seeded with an average cell density of one cell per well in 200 µL cell culture medium and incubated at 37°C and 5% CO 2. All conditions contained 6 - 8 mM L-glutamine. Cloning efï¬ciencies in various protein-free cell culture media with (blue) or without (grey) addition of ClonaCell™-CHO ACF Supplement or in DMEM + 10% FBS (orange bar) are shown. Error bars represent the standard deviation based on triplicate data for each condition, except Medium 2 (both with and without ClonaCell™-CHO ACF Supplement), for which the average of duplicate data is shown. Medium 1 = ClonaCell™-CHO CD Liquid Medium, Medium 2 = Gibco® CD CHO Medium, Medium 3 = Gibco® CD OptiCHO™ Medium, Medium 4 = Lonza PowerCHO™2.