mTeSR™3D_产品中心_北京砺达辰生物科技有限公司

细胞培养基及添加物

mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

概要

技术资料

数据及文献

概要

mTeSR™3D was developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. The novel fed-batch workflow saves time and media. Daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.

mTeSR™3D is a specialised and serum-free formulation. This culture system is compatible with a range of suspension culture vessels.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	mTeSR™3D	03950	All	English
Manual	mTeSR™3D	03950	All	English
Safety Data Sheet 1	mTeSR™3D	03950	All	English
Safety Data Sheet 2	mTeSR™3D	03950	All	English
Safety Data Sheet 3	mTeSR™3D	03950	All	English
Safety Data Sheet 4	mTeSR™3D	03950	All	English

数据及文献

Data

Figure 1. hPSC growth in mTeSR™3D

mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

Figure 2. hPSC aggregate morphology in mTeSR3D

Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.

Figure 3. Expression of undifferentiated cell markers

hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

Figure 4. Tri-lineage Differentiation

hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).