StemSpan™ Myeloid Expansion Supplement II (100X)_产品中心

细胞培养基及添加物

StemSpan™ Myeloid Expansion Supplement II (100X)

Serum-free culture supplement for expansion and differentiation of human monocytes

概要

技术资料

数据及文献

概要

StemSpan™ Myeloid Expansion Supplement II (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD14+ monocytes from CD34+ cells isolated from human cord blood (CB) samples.

StemSpan™ Myeloid Expansion Supplement II (100X) is intended for use in combination with any of the following StemSpan™ media:
• StemSpan™ SFEM (Catalog #09600)
• StemSpan™ SFEM II (Catalog #09605)
• StemSpan™-XF (Catalog #100-0073)
• StemSpan™-AOF (Catalog #100-0130)

When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement II typically promotes the production of hundreds of CD14+ monocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	StemSpan™ Myeloid Expansion Supplement II (100X)	02694	All	English
Safety Data Sheet	StemSpan™ Myeloid Expansion Supplement II (100X)	02694	All	English

数据及文献

Data

Figure 1. Production of CD14+ Monocytes by the Expansion and Lineage-Specific Differentiation of Human CB CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement II

Flow cytometry dot plots for 14-day cultures of human CB CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement II. The plots show expression of CD34 and CD45 (A) before and (B) after culture, and (C) expression of CD13 and CD14 on the expanded cells. The frequency of CD34+ cells declined from 87% before culture to less than 1% after 14 days. CD14+ monocytes increased from < 5% on day 0 to 90% by day 14.