STEMdiff™ Ventral Forebrain Organoid Differentiation Kit_产品中心

细胞培养基及添加物

STEMdiff™ Ventral Forebrain Organoid Differentiation Kit

Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells

概要

技术资料

数据及文献

概要

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding. STEMdiff™ Dorsal and Ventral Forebrain Organoid Differentiation Kits are serum-free cell culture media that work with AggreWell™-generated embryoid bodies to prevent organoid fusion and enable the scalable generation of over 500 highly reproducible organoids per kit. Adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017), these brain-region-specific organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the developing human forebrain. STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (Catalog #08620) generates tissue of the early developing dorsal pallium, while STEMdiff™ Ventral Forebrain Organoid Differentiation Kit (Catalog #08630) generates tissue of the early developing ventral subpallium. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017). For extended periods of organoid culture (> 50 days), the components required for organoid maintenance are available as STEMdiff™ Neural Organoid Maintenance Kit (Catalog #100-0120).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English
Safety Data Sheet 1	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English
Safety Data Sheet 2	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English
Safety Data Sheet 3	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English
Safety Data Sheet 4	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English
Safety Data Sheet 5	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English
Safety Data Sheet 6	STEMdiff™ Ventral Forebrain Organoid Differentiation Kit	08630	All	English

数据及文献

Data

Schematic protocol diagram to generate patterned ventral forebrain organoids from iPSCs

Figure 1. Schematic for the STEMdiff™ Ventral Forebrain Organoid Differentiation Kit

Human ES or iPS cell-derived ventral forebrain organoids can be generated in 43 days. Embryoid bodies can be created in 6 days with AggreWell™800 plates. The EBs are then cultured in suspension, allowing growth and subsequent patterning to the ventral forebrain. For the long-term maintenance and further maturation of ventral forebrain organoids, see the PIS.

Figure 2. Fluorescent Imaging in BrainPhys™ Imaging Optimized Medium Improves Signal-to-Background Ratios of 3D Neural Cultures

GFP-labeled ventral forebrain organoids were co-cultured and fused with unlabeled dorsal forebrain organoids for one week prior to live imaging in Forebrain Organoid Differentiation Medium from STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (right) or BrainPhys™ Imaging Optimized Medium (BPI, left). Interneuron migration can be visualized more clearly in BPI. Adapted from Zabolocki et al. 2020, Nature Communications, available under a Creative Commons 4.0 License.