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细胞培养基及添加物

STEMdiff™ Neural Crest Differentiation Kit

Cell culture kit for establishment of hPSC-derived neural crest cells

概要
技术资料
数据及文献

概要

STEMdiff™ Neural Crest Differentiation Kit creates a serum-free medium for differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to neural crest cells. These neural crest cells, which are characterized by neural crest markers such as SOX10 and CD271, can be differentiated to several downstream derivatives including chondrocytes, osteoblasts, and peripheral neurons. This medium is compatible with human ES and iPS cells maintained in either mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #05825) or TeSR™-E8™ (Catalog #05990).

数据及文献

Data

Figure 1.

Neural crest cells are produced using STEMdiff™ Neural Crest Differentiation Kit after 6 days in culture. For information on continued passage of neural crest cells, contact us at https://www.stemcell.com/products/product-types/cell-culture-media-and-supplements/techsupport@stemcell.com

STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

Figure 2. STEMdiff™ Neural Crest Differentiation Kit Generates a Highly Pure Population of NCCs with Minimal CNS-type Progenitors

After 6 days in culture, neural crest cells (NCCs; SOX10+, red; CD271+, light blue) outnumber CNS-type progenitors (PAX6+, green). (A) Channel merge of cells fixed 2 days after being passaged on day 6. Individual immunofluorescence channels show (B) DAPI, (C) PAX6, (D) SOX10, and (E) CD271. Scale bar = 100 μm.

NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

Figure 3. NCCs Generated with the STEMdiff™ Neural Crest Differentiation Kit Are Multipotent

NCCs (A) were cultured for 6 days and display typical morphology. (B) Culturing NCCs using established protocols generates peripheral neurons (Peripherin, green; BRN3a, red; DAPI, blue). (C) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™-ACF Chondrogenic Differentiation Kit generates a chondrocyte pellet (Alcian Blue, Nuclear Fast Red) with deposition of cartilage around the cells. (D) Passaging NCCs into MesenCult™-ACF Plus Medium and then into the MesenCult™ Osteogenic Differentiation Kit (Human) generates an osteoblast culture with high levels of alkaline phosphatase-positive mineral deposition. Scale bar = 500 μm (A-C), 1 mm (D).