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概要
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) is a complete, serum- and animal component-free medium and supplement kit that supports highly efficient differentiation of human ES cells and iPS cells that have been maintained in TeSR™-E8™ to definitive endoderm. Cells differentiated using the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) express high levels of endoderm markers, including CD184 (CXCR4) and SOX17. When used in combination with Vitronectin XF™, the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) provides a completely xeno-free workflow for the generation of multipotent definitive endoderm.
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) | 05115 | Component #05113: Lot 18D89658 and higher | English |
| Safety Data Sheet 1 | STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) | 05115 | All | English |
| Safety Data Sheet 2 | STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) | 05115 | All | English |
| Safety Data Sheet 3 | STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) | 05115 | All | English |
| Safety Data Sheet 4 | STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) | 05115 | All | English |
| Safety Data Sheet 5 | STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) | 05115 | All | English |
数据及文献
Data
Figure 1. Definitive Endoderm Differentiation is Efficient Across Multiple ES and iPS Cell Lines Maintained in Either mTeSR™1 or TeSR™-E8™ Maintenance Media
Quantitative analysis of definitive endoderm formation in multiple hES (H1 and H9) and hiPS (WLS-4D1 and STiPS-M001) lines as measured by co-expression of CXCR4 and SOX17. Cells maintained in mTeSR™1 or TeSR™-E8™ were differentiated using the STEMdiff™ Definitive Endoderm Kit or the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™-Optimized), respectively. Data are expressed as the mean percentage of cells expressing both markers. Error bars indicate SEM.
