Anti-Phospho-SHIP Antibody, Polyclonal_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Phospho-SHIP Antibody, Polyclonal

Rabbit polyclonal antibody against human, mouse phosphoSHIP

概要

技术资料

数据及文献

概要

Phospho-SHIP is a highly phosphorylated form of SHIP. SH2-containing inositol phosphatase (SHIP) is a 145 kDa hematopoietic-restricted protein that becomes tyrosine-phosphorylated and associated with the adaptor protein, Shc, following cytokine, growth factor, chemokine, and immunoreceptor stimulation. SHIP also hydrolyzes the critical phosphatidylinositol (PI)-3-kinase (PI3K)-generated second messenger, PI-3,4,5-P3 (PIP3), to PI-3,4-P2 1,2 and therefore acts as an important negative regulator of the PI3K pathway. This antibody reacts with mouse and human tyrosine-phosphorylated SHIP.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Phospho-SHIP Antibody, Polyclonal	60142	All	English
Safety Data Sheet	Anti-Phospho-SHIP Antibody, Polyclonal	60142	All	English

数据及文献

Data

(A) Flow cytometry analysis of bone marrow derived mast cells (BMMCs) from normal (+/+) or SHIP knockout (-/-) mice. Cells were stimulated with stem cell factor (SCF; filled histogram) or left unstimulated (solid line historgram), and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(B) BMMCs were either stimulated with SCF or left unstimulated, and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(C) Western blot analysis of total cell lysates from human TF-1 cells stimulated with IL-3, mouse WEHI-231 B cells stimulated with anti-IgM, and mouse BMMCs stimulated with SCF, and probed with Anti-Phospho-SHIP Antibody, Polyclonal. The blot was re-probed with an anti-SHIP antibody to show equal loading of unstimulated (C) and stimulated (S) samples. Phospho-SHIP has a predicted molecular mass of 145 kDa.