Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136_产品中心_北京砺达辰生物科技有限公司

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Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136

(C3H x BALB/c) F1 hybrid monoclonal IgG2a antibody against mouse NK1.1 (CD161)

概要

技术资料

数据及文献

概要

The PK136 antibody reacts with murine NK1.1 (CD161), an ~80 kDa homodimeric type 2 transmembrane glycoprotein and C-type lectin receptor expressed on NK cells, NK-T cells, and rare subsets of T cells and monocytes in select strains of mice. NK1.1 is encoded by the Klrb1b and Klrb1c genes, which specify CD161b and CD161c polypeptides, respectively. CD161b is expressed only by Swiss NIH and SJL mice, and CD161c by certain strains such as C57BL, FVB/N, and NZB (but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, or 129). NK1.1 has functional roles in modulating several processes, including the activation and proliferation of NK cells, induction of interferon-γ production, and release of cytotoxic granules. Its expression on the cell surface is specifically upregulated by IL-12. For detection of NK cells in NK1.1- strains, Anti-Mouse CD49b Antibody, Clone DX5 (Catalog #60020) is recommended. DX5 recognizes CD49b (integrin α2), another commonly used NK cell marker.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488	60103AD, 60103AD.1	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC	60103AZ, 60103AZ.1	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin	60103BT, 100-0462, 100-0463	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC	60103FI, 100-0464, 100-0465	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5	60103PS, 60103PS.1	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™	60103PB, 60103PB.1	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136	100-0459	All	English
Product Information Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE	100-0460, 100-0461	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488	60103AD, 60103AD.1	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC	60103AZ, 60103AZ.1	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin	60103BT, 100-0462, 100-0463	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC	60103FI, 100-0464, 100-0465	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5	60103PS, 60103PS.1	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136	100-0459	All	English
Safety Data Sheet	Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE	100-0460, 100-0461	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, APC (Catalog #60071AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, Biotin (Catalog #60071BT), followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, FITC (Catalog #60071FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a mouse IgG2a, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.