Anti-Mouse Ly-6G Antibody, Clone 1A8_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse Ly-6G Antibody, Clone 1A8

Rat monoclonal IgG2a antibody against mouse Ly-6G

概要

技术资料

数据及文献

概要

The 1A8 antibody reacts with Ly-6G, a 21 - 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery, Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8	60031	All	English
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, APC	60031AZ, 60031AZ.1	All	English
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin	60031BT, 60031BT.1	All	English
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC	60031FI, 60031FI.1	All	English
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, PE	60031PE, 60031PE.1	All	English
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5	60031PS, 60031PS.1	All	English
Product Information Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™	60031PB, 60031PB.1	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8	60031	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, APC	60031AZ, 60031AZ.1	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin	60031BT, 60031BT.1	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC	60031FI, 60031FI.1	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, PE	60031PE, 60031PE.1	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5	60031PS, 60031PS.1	All	English
Safety Data Sheet	Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™	60031PB, 60031PB.1	All	English

数据及文献

Data

Figure 1. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). (B) Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse uterine tissue labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by anti-mouse, HRP. DAB substrate was used for visualization.

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).