Anti-Mouse Gr-1 Antibody, Clone RB6-8C5_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse Gr-1 Antibody, Clone RB6-8C5

Rat monoclonal IgG2b antibody against mouse Gr-1 (Ly-6G/Ly-6C)

概要

技术资料

数据及文献

概要

The RB6-8C5 antibody reacts with the structurally related murine Ly-6G (21 - 25 kDa) and Ly-6C (14 - 16 kDa) GPI-anchored proteins, which together comprise the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils, subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. Whereas the RB6-8C5 binds to both Ly-6G and Ly-6C, another commonly used anti-Gr-1 antibody, clone 1A8, binds specifically to Ly-6G. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes and dendritic cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5	60028	All	English
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Alexa Fluor® 488	60028AD, 60028AD.1	All	English
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC	60028AZ, 60028AZ.1	All	English
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin	60028BT, 60028BT.1	All	English
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC	60028FI, 60028FI.1	All	English
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE	60028PE, 60028PE.1	All	English
Product Information Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5	60028PS	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5	60028	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Alexa Fluor® 488	60028AD, 60028AD.1	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC	60028AZ, 60028AZ.1	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin	60028BT, 60028BT.1	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC	60028FI, 60028FI.1	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE	60028PE, 60028PE.1	All	English
Safety Data Sheet	Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5	60028PS	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2b, kappa isotype control antibody, followed by SAV APC (solid line histogram).

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure 4. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a rat IgG2b, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC is shown (solid line histogram).

Figure 5. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Figure 7. Data for PerCP-Cy5.5-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on granulocytes) labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).