Anti-Mouse F4/80 Antibody, Clone BM8_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse F4/80 Antibody, Clone BM8

Rat monoclonal IgG2a antibody against human, mouse F4/80

概要

技术资料

数据及文献

概要

The BM8 antibody reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. F4/80 is considered a marker of choice for the identification of mature tissue macrophages, being broadly but variably expressed by this cell type in the liver (Kupffer cells), skin (Langerhans cells), bone marrow stroma, pancreas, thymus, spleen (red pulp), lung, and other tissues. It is also expressed by circulating monocytes, eosinophils and a subset of dendritic cells. F4/80 expression levels increase following activation of macrophages. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8	60027	All	English
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488	60027AD, 60027AD.1	All	English
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, APC	60027AZ, 60027AZ.1	All	English
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, Biotin	60027BT, 60027BT.1	All	English
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, FITC	60027FI, 60027FI.1	All	English
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, PE	60027PE, 60027PE.1	All	English
Product Information Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5	60027PS, 60027PS.1	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8	60027	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488	60027AD, 60027AD.1	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, APC	60027AZ, 60027AZ.1	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, Biotin	60027BT, 60027BT.1	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, FITC	60027FI, 60027FI.1	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, PE	60027PE, 60027PE.1	All	English
Safety Data Sheet	Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5	60027PS, 60027PS.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit and labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488.
(C) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody and antimouse CD45 APC.

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa PE isotype control antibody and anti-mouse CD45 APC.

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.